鸡肝细胞中L-BABP基因表达对脂类代谢基因及甘油三酯和总胆固醇的影响

doi:10.11843/j.issn.0366-6964.2015.01.005

Abstract

Abstract:

This study was designed to investigate the role of L-BABP gene in lipid metabolism of hepatocytes and the effect of L-BABP gene on the genes related to lipid metabolism in chicken.L-BABP gene expression was downregulated by siRNA interference and upregulated via overexpression plasmids method.The genes expression level in chicken hepatocytes were detected at 24，36，48，60 and 72 h after transfection with the siRNAs and overexpression plasmids of L-BABP.The results showed that APOAΙ，APOB，PERILIPIN gene expression levels，the amount of cholesterol and triglyceride in the medium are significantly affected by L-BABP gene expression changes.Therefore，L-BABP may involve in fat deposition，metabolism of total cholesterol and the process of lipolysis lipid metabolism.

CLC Number:

S831.2

GAO Guang-liang，ZHANG Qing-qiu，LI Hui，WANG Qi-gui. The Effect of L-BABP Gene Expression on the Lipid Metabolism Genes and Cholesterol and Triglyceride in Chicken Hepatocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 32-40.

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The Effect of L-BABP Gene Expression on the Lipid Metabolism Genes and Cholesterol and Triglyceride in Chicken Hepatocytes

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